Monash Histology Platform

Monash Histology Platform (MHP) is a world class, full service histology platform located in Melbourne. The platform provides full end to end Histology services OR part thereof, as well as access to equipment for “Do it yourself" work including paraffin embeddors, microtomes, cryostats, laser microdissection, and routine staining areas. The Histology Platform operates on a fee for service basis for researchers, academic staff, students and clinical collaborators. Our 3 nodes in Melbourne are located at Monash Clayton campus, Hudson Institute and Alfred Hospital. A full consultation and collaboration service, expert training in all histology techniques, specialist methacrylate and epoxy resin methods, microscopic and digital imaging, tissue micro-array generation and automated immunohistochemical and multiplex immunofluorescence techniques are also available.

Please visit our website for more information or submit an enquiry to see how we can help you today!

Monash Histology staff can provide training and consultation to clients for studies and projects that involve OCT embedded frozen tissue sectioning. To meet a broad range of frozen tissue preparation requirements, for both fresh and fixed tissue, the Platform offers professional service and do-it-yourself access to cryostats. Along with experienced staff and our versatile Leica cryostats equipped with a programmable trimming and sectioning option, we can cut sections from 0.5 to 300 microns thickness. A Leica autostainer XL unit with coverslipper is also offered as part of our services for routine H&E and modified special stains. The Platform at each node also offers equipment and areas for ‘do it yourself’ OCT embedding, cryotomy sectioning and routine and special staining. 

Please visit our website for more information or submit an enquiry to see how we can help you today!

Monash Histology Platform has two state-of-the-art Aperio Slide Scanning Units and a Olympus VS120 scanner. Our Platform offers services for all your scanning requirements, and provides an imaging service to clients wishing to utilise data scans from brightfield or fluorescent slides. The Aperio Scanscope AT Turbo can scan up to 400 brightfield slides at a time. The Aperio Scanscope FL can scan up to 5 fluorescent slides per run. The Olympus VS120 has a duel brightfield and fluorescent scanning capabilities and can scan up to 100 slides. Images are stored on a central server before being accessed by the client. Image analysis software is provided free of charge for the client to utilize at their own computer.

Both the Aperio ScanScope AT Turbo and FL systems have interactive console software that allows customisation of scans. The eSlide Manager Server and ImageScope software facilitate annotation and analysis of images, and functions as a digital slide management system. Integration of both Brightfield and Fluorescent scanning services into the workflows of MHP has provided Clients with substantial advantages over conventional microscopy.

Please visit our website for more information or submit an enquiry to see how we can help you today!

Immunohistochemistry is used to detect antigens in tissue sections and enable visualisation using brightfield, fluorescence, or electron microscopy. Detection is performed using an enzyme (Horseradish peroxidase-HRP , alkaline phosphatase) combined with a chromogenic substrate (e.g. DAB, Permanent Red, AEC), a fluorophore (e.g. Alexa Fluor dye family) or a metal for electron microscopic visualisation (e.g. gold).

At Monash Histology Platform we employ use of DAKO PT Link antigen retrieval combined with DAKO immunohistochemistry autostainer to optimise primary antibodies and run IHC experiments that includes in-vivo or in-vitro experiments, DAB chromogen or Fluorescent labelled staining and imaging. The flexible settings on these equipment makes it possible to run multiplex immunofluorescence staining and multiple antibody test to save both time and resources. There are 5 base protocols on the Autostainer Plus, which were created for use: Monash Routine, Monash Fluorescent, Monash Mouse-on-Mouse, Monash G|2 Dako Doublestain system, and the Monash TUNEL protocol. 

Please visit our website for more information or submit an enquiry to see how we can help you today!

Our paraffin laboratory offers professional Histology services for preparation of microscopic sections from a wide variety of samples onto slides. This includes pre-processing, dissection and preparation of specimens as well as processing to paraffin with the Leica Peloris, Leica ASP300s and Tissue Tek VIP2000 tissue processors. A Leica autostainer XL unit with coverslipper is also offered as part of our services for routine H&E and modified special stains. The Platform at each node also offers equipment and areas for ‘do it yourself’ embedding, microtomy sectioning, routine and special staining. 

Please visit our website for more information or submit an enquiry to see how we can help you today!

The Resin laboratory provides specialist processing, sectioning and staining services for projects requiring resin sections. The laboratory is well equipped with specialised microtomes including Leica RM2165, Leica Ultracut microtomes for cutting both methacrylate and epoxy resins, Leica RM2265 microtome with a stereomicroscope and fully equipped staining area including an automated staining unit with coverslipper. 

Our fully programmable, motorised rotary microtomes used for cutting resin (GMA) produces consistent and high quality sections. We also provide automatated staining service for GMA slides such as Haematoxylin and Eosin (H&E) Periodic Acid and Schiff's (PAS).

Cutting thick tissue sections with our Leica Vibratome maintains the morphology, enzyme activity and cell viability of the tissue. The vibration, amplitude, speed and angle of the blade can be controlled to be able to generate thick sections from 40 to 1000 um from samples. The Vibratome sectioning also minimizes artefacts, compression distortion, cell destruction and other inherent artefact effects of sectioning.

Histology staff can prepare thick fresh and unfixed sections as part of our professional service and also provide training to academic researchers and students for do-it-yourself use. The thick slices can also be prepared from Golgi stained tissue and also for organotypic slice tissue culture experiments that includes for in-vivo or in-vitro experiments, staining or imaging.

Please visit our website for more information or submit an enquiry to see how we can help you today!

Monash Histology Platform (MHP) is a world class, full service histology platform located in Melbourne. The platform provides full end to end Histology services OR part thereof, as well as access to equipment for “Do it yourself" work including paraffin embeddors, microtomes, cryostats, laser microdissection, and routine staining areas. The Histology Platform operates on a fee for service basis for researchers, academic staff, students and clinical collaborators. Our 3 nodes in Melbourne are located at Monash Clayton campus, Hudson Institute and Alfred Hospital. A full consultation and collaboration service, expert training in all histology techniques, specialist methacrylate and epoxy resin methods, microscopic and digital imaging, tissue micro-array generation and automated immunohistochemical and multiplex immunofluorescence techniques are also available.

Please visit our website for more information or submit an enquiry to see how we can help you today!

Monash Histology staff can provide training and consultation to clients for studies and projects that involve OCT embedded frozen tissue sectioning. To meet a broad range of frozen tissue preparation requirements, for both fresh and fixed tissue, the Platform offers professional service and do-it-yourself access to cryostats. Along with experienced staff and our versatile Leica cryostats equipped with a programmable trimming and sectioning option, we can cut sections from 0.5 to 300 microns thickness. A Leica autostainer XL unit with coverslipper is also offered as part of our services for routine H&E and modified special stains. The Platform at each node also offers equipment and areas for ‘do it yourself’ OCT embedding, cryotomy sectioning and routine and special staining. 

Please visit our website for more information or submit an enquiry to see how we can help you today!

Monash Histology Platform has two state-of-the-art Aperio Slide Scanning Units and a Olympus VS120 scanner. Our Platform offers services for all your scanning requirements, and provides an imaging service to clients wishing to utilise data scans from brightfield or fluorescent slides. The Aperio Scanscope AT Turbo can scan up to 400 brightfield slides at a time. The Aperio Scanscope FL can scan up to 5 fluorescent slides per run. The Olympus VS120 has a duel brightfield and fluorescent scanning capabilities and can scan up to 100 slides. Images are stored on a central server before being accessed by the client. Image analysis software is provided free of charge for the client to utilize at their own computer.

Both the Aperio ScanScope AT Turbo and FL systems have interactive console software that allows customisation of scans. The eSlide Manager Server and ImageScope software facilitate annotation and analysis of images, and functions as a digital slide management system. Integration of both Brightfield and Fluorescent scanning services into the workflows of MHP has provided Clients with substantial advantages over conventional microscopy.

Please visit our website for more information or submit an enquiry to see how we can help you today!

Immunohistochemistry is used to detect antigens in tissue sections and enable visualisation using brightfield, fluorescence, or electron microscopy. Detection is performed using an enzyme (Horseradish peroxidase-HRP , alkaline phosphatase) combined with a chromogenic substrate (e.g. DAB, Permanent Red, AEC), a fluorophore (e.g. Alexa Fluor dye family) or a metal for electron microscopic visualisation (e.g. gold).

At Monash Histology Platform we employ use of DAKO PT Link antigen retrieval combined with DAKO immunohistochemistry autostainer to optimise primary antibodies and run IHC experiments that includes in-vivo or in-vitro experiments, DAB chromogen or Fluorescent labelled staining and imaging. The flexible settings on these equipment makes it possible to run multiplex immunofluorescence staining and multiple antibody test to save both time and resources. There are 5 base protocols on the Autostainer Plus, which were created for use: Monash Routine, Monash Fluorescent, Monash Mouse-on-Mouse, Monash G|2 Dako Doublestain system, and the Monash TUNEL protocol. 

Please visit our website for more information or submit an enquiry to see how we can help you today!

Our paraffin laboratory offers professional Histology services for preparation of microscopic sections from a wide variety of samples onto slides. This includes pre-processing, dissection and preparation of specimens as well as processing to paraffin with the Leica Peloris, Leica ASP300s and Tissue Tek VIP2000 tissue processors. A Leica autostainer XL unit with coverslipper is also offered as part of our services for routine H&E and modified special stains. The Platform at each node also offers equipment and areas for ‘do it yourself’ embedding, microtomy sectioning, routine and special staining. 

Please visit our website for more information or submit an enquiry to see how we can help you today!

The Resin laboratory provides specialist processing, sectioning and staining services for projects requiring resin sections. The laboratory is well equipped with specialised microtomes including Leica RM2165, Leica Ultracut microtomes for cutting both methacrylate and epoxy resins, Leica RM2265 microtome with a stereomicroscope and fully equipped staining area including an automated staining unit with coverslipper. 

Our fully programmable, motorised rotary microtomes used for cutting resin (GMA) produces consistent and high quality sections. We also provide automatated staining service for GMA slides such as Haematoxylin and Eosin (H&E) Periodic Acid and Schiff's (PAS).

Cutting thick tissue sections with our Leica Vibratome maintains the morphology, enzyme activity and cell viability of the tissue. The vibration, amplitude, speed and angle of the blade can be controlled to be able to generate thick sections from 40 to 1000 um from samples. The Vibratome sectioning also minimizes artefacts, compression distortion, cell destruction and other inherent artefact effects of sectioning.

Histology staff can prepare thick fresh and unfixed sections as part of our professional service and also provide training to academic researchers and students for do-it-yourself use. The thick slices can also be prepared from Golgi stained tissue and also for organotypic slice tissue culture experiments that includes for in-vivo or in-vitro experiments, staining or imaging.

Please visit our website for more information or submit an enquiry to see how we can help you today!