Monash Micro Imaging - MHTP

GETTING STARTED:

New Users

All new users, or those who have not used iLab for MMI-MHTP before, must register with MMI-MHTP via: 'Request Services' tab > MMI-MHTP Registration for New Users > initiate request

Existing Users

To submit instrument bookings: 'Schedule Equipment' tab > select preferred instrument > View Schedule > select preferred time slot

 

Overview of Services

MMI is a central university research Platform providing advanced optical microscopy infrastructure, analytical support and training for the biomedical and life sciences. Monash University is partnering with the Hudson Institute of Medical Research to establish world-class technology research platforms at the Monash Health Translation Precinct. These are made up of core facilities and capabilities that provide high-quality specialist research services (see http://www.monash.edu/research/infrastructure/platforms).

Our core technology research platforms are coordinated through the Office of the Vice-Provost (Research and Research Infrastructure).

 

MMI Platform Vision

Monash Micro Imaging strives to provide a fully integrated optical microscopy imaging research environment for the biomedical and life sciences. Our aim is to empower researchers by providing access to state-of-the-art microscopy technology, and to ensure excellence in imaging through expertise in experimental design, application development, data analysis and research collaboration.

 

MMI Platform Mission

To provide world class microscopy instrumentation for your research needs

To provide substantive, expert support for cellular and subcellular microscopy, associated techniques and data analysis

To provide excellence in research training in microscopy

To collaborate in ground breaking biomedical/life science research and innovate new imaging approaches

 

To learn more about MMI please visit:

https://platforms.monash.edu/mmi/

http://hudson.org.au/facility/micro-imaging/

 

Platform Staff

 

Dr. Sarah Creed 

Facility Manager and Optical Microscopy Specialist

+61 3 8572 2816

sarah.creed@hudson.org.au

  

Dr. Steve Mercer 

Optical Microscopist

+61 3 8572 2790

steve.mercer@hudson.org.au

 

Location and Hours of Operation

Hours Location

Facility Hours: 24/7

Staff Hours: 8:00am-5:00pm, Monday-Friday 

         4R.45, Level 4 Building 258 TRF

         The Hudson Institute of Medical Research       

         27-31 Wright St.

         Clayton, VIC 3168

Links and Resources

  1. http://hudson.org.au/facility/micro-imaging/
  2. http://platforms.monash.edu/mmi

Contacts

Name Role Phone Email Location
Dr. Sarah Creed
Facility Manager and Optical Microscopy Specialist
 
+61 3 8572 2816
 
sarah.creed@hudson.org.au
 
4R.44, Building 258 TRF, The Hudson Institute of Medical Research, 27-31 Wright St., Clayton VIC 3168
 
Dr. Steve Mercer
Optical Microscopist
 
+61 3 8572 2790
 
steve.mercer@hudson.org.au
 
4R.44, Building 258 TRF, The Hudson Institute of Medical Research, 27-31 Wright St, Clayton VIC 3168
 

Service list


Search available services:
Name Description Price
Key Instrumentation and Experties

Monash Micro Imaging (MMI) is world-class in optical imaging. We have core facilities at the Clayton campus and specialised nodes at the Monash Health Translation Precinct and the Alfred Research Alliance. MMI technologies include advanced light microscopy, fluorescence and confocal microscopy, multiphoton microscopy, super-resolution microscopy, light-sheet microscopy, which cater to a diverse range of morphological and functional  characterisation in the life sciences. All technologies are underpinned by bioimage analysis and research training.

 

Key Instrumentation

 

Our instrumentation is sourced from major innovative microscope companies, including Leica, Zeiss, Olympus, Nikon, Abberior, LaVision Biotec, and Intelligent Imaging Innovations (3i).

 

  • Widefield fluorescence microscopy
  • Polarisation microscopy
  • Confocal microscopy
  • Multiphoton microscopy 
  • Deconvolution microscopy
  • Stereology (at MHTP)
  • Super-resolution Microscopy: Zeiss AiryScan, Leica Lightning and Thunder Deconvolution, and STED
  • Lightsheet microscopy including Lattice Lightsheet and Ultramicroscopy
  • Dynamic Fluorescence analysis: FRAP, FLIM, FCS, photoactivation, ratiometric approaches
  • Image analysis: ImageJ/FIJI, Metamorph, CellSens, LASAF, NIS elements, SymPhoTime (FLIM), Imaris Suite, Huygens Core/Professional, Drishti.

Experties 

 

Our team provide expertise and training across a wide range of analytical microscopes and microscopy modalities in the biomedical and life sciences. Our services range in complexity from sample preparation and labelling for fluorescence analysis to performing live-cell experiments for cell signalling and organ/organism development. We provide guidance and training to allow scientists and students to undertake cutting edge analytical research with confidence. Our platform works closely with other MTRPs, such as Histology, Cryo-EM and eResearch, to provide integrated microscopy support to a large research community.

 

Working with us

 

  • Fee for access
  • Training
  • Collaborative research
Inquire
Specialist Services

Our team provides advanced microscopy instrumentation and analytical techniques to a large research community. Ranging in complexity from the simple labelling and mounting of slides for immunofluorescence microscopy, to live imaging in multiwell plates or sophisticated perfusion chambers, we will guide and train you to perform experiments, produce high-quality images and extract analytical data.

 

Advanced light and fluorescence microscopy

 

Our instrumentation provides a solid platform of advanced light and fluorescence microscopy techniques, including automation, highspeed imaging, time-lapse, slide scanning (in conjunction with our Histology Platform) and image tiling, and live-cell imaging on slides, chambers or microplates. Both upright and inverted instruments are available, and all systems are supported by a comprehensive range of professional software for bioimage analysis to provide quantitative results.

 

 

Live-cell  imaging  is one of our specialities

 

Most of our instruments are equipped with live- cell incubators, specialised cell chambers or multiwell plates that support live and long term imaging experiments. We have extensive knowledge in experimental design, labelling and analysis.

 

 

Optical  sectioning and 3D analysis

 

Our range of instrument modalities includes confocal (laser scanning and spinning disk) and multiphoton microscopes. For high-speed imaging, we have resonant scanning confocal and light-sheet microscopes. Imaging deeper into tissue can also be done by multiphoton imaging in live, fixed or cleared tissue microscopy which is capable of imaging to a depth of 2-6mm with specialised objectives.

 

 

Special methods and emerging technologies

 

Our expert staff offer extensive collaborative support for the more novel or complex instruments and applications, including Fluorescence Lifetime Imaging Microscopy (FLIM), Lattice Lightsheet Microscopy, Stimulated Emission Depletion Microscopy (STED), birefringence microscopy, AiryScan, Deconvolution microscopy(from both widefield and confocal sources) and, in conjunction with the Cryo-EM platform, correlative light and electron microscopy.

 

 

Image analytics  and data handling

 

Extracting and understanding bioimaging data is crucial, and handling big datasets is often a bottleneck in research. Our Staff are available to train scientists and students in the analytical software we licence (ImageJ/FIJI, Imaris, Huygens, Metamorph). In conjunction with eResearch, we are also building data handling and analysis pipelines to facilitate the flow of (big)data from instrument to computational workspaces and ultimately to publication.

 

Inquire