Established in 2016, the Metabolic Phenotyping Platform provides Monash researchers, as well as academic and commercial organisations, with access to experts, technologies and techniques to perform the highest quality in vivo metabolic phenotyping procedures. We offer a broad range of procedures for full metabolic characterisation across many physiological conditions. Our standardised methods for in vivo testing are in line with world leading centres, allowing for high-throughput metabolic phenotyping procedures to fast-track research outcomes. Key services and instrumentation include body composition analysis, non-invasive metabolic monitoring, food and liquid intake monitoring, first line phenotyping, surgical procedures, and gold standard metabolic phenotyping.
At the Monash Metabolic Phenotyping Facility, our gold standard metabolic phenotyping tests combine arterial and jugular vein cannulation with radioisotopes to look at whole body insulin action, glucose sensitivity and the metabolic response to treadmill exercise. In addition, the rates of glucose and/or fatty acid uptake by multiple tissue can be assessed.
All tests are performed in conscious and unrestrained mice. These are considered to be the gold standard for examining metabolism in vivo. We can undertake several procedures on your behalf, including:
Hyperinsulinaemic-euglycaemic clamp: assesses whole body insulin action and the ability of insulin to suppress hepatic glucose production. Rates of glucose uptake by multiple tissues can also be assessed.
Hyperglycaemic clamp: assesses blood metabolite and hormone responses to an acute elevation in blood glucose levels.
Hyperinsulinaemic-hypoglycaemic clamp: assesses the counter regulatory response to an acute drop in blood glucose levels via insulin.
Metabolic response to exercise: assesses endogenous glucose production and/or rates of glucose uptake by multiple tissues.
Should you require us to perform a procedure not listed here, we are more than happy to discuss your requirements and the feasibility of performing your procedure through our facility.
Inquire
First Line Phenotyping
The Monash Metabolic Phenotyping Facility can undertake a number of first line phenotyping procedures. Our experienced staff follow standardised procedures that are in line with world leading facilities, are similar to those performed in humans and precede the more complex gold standard in vivo metabolic studies. We can undertake several procedures on your behalf, including:
Body composition analysis (body weight, lean mass, fat mass, bone density).
Whole body glucose metabolism, insulin sensitivity and glucose production.
Exercise endurance and/or exercise capacity ( including gas exchange data)
Pharmacokinetic and half-life characterisation.
Behavioural analysis.
Investigators can utilise our surgical procedures and have the option of choosing one test or a combination of tests to enhance the level of sensitivity for your research project. Should you require us to perform a procedure not listed here, we are more than happy to discuss your requirements and the feasibility of performing your procedure through our facility
Inquire
Surgical Procedures
The Monash Metabolic Phenotyping Facility can perform a wide range of surgical procedures. Our experienced staff follow standardised procedures that are in line with world leading facilities, allowing for high-throughput of complex procedures, such as:
Ovariectomy
Castration
Splenectomy
Osmotic mini pump or pellet installation
Portal Vein Cannulation
Jugular vein cannulation, allowing for intravenous infusion in conscious and unrestrained mice.
Carotid artery cannulation, allowing for arterial blood sampling in conscious and unrestrained mice.
Dual cannulation (carotid artery and jugular vein), allowing for simultaneous arterial blood sampling and intravenous infusion in conscious and unrestrained mice.
Technology and methodological development is a primary goal of the Monash Metabolic Phenotyping Facility. Thus, should you require us to perform a surgical procedure not listed here, we are more than happy to discuss your requirements and the feasibility of performing this through our facility.
The Monash Metabolic Phenotyping Facility can perform a wide range of surgical procedures. Our experienced staff follow standardised procedures that are in line with world leading facilities, allowing for high-throughput of complex procedures, such as:
Ovariectomy
Castration
Splenectomy
Osmotic mini pump or pellet installation
Portal Vein Cannulation
Jugular vein cannulation, allowing for intravenous infusion in conscious and unrestrained mice.
Carotid artery cannulation, allowing for arterial blood sampling in conscious and unrestrained mice.
Dual cannulation (carotid artery and jugular vein), allowing for simultaneous arterial blood sampling and intravenous infusion in conscious and unrestrained mice.
Technology and methodological development is a primary goal of the Monash Metabolic Phenotyping Facility. Thus, should you require us to perform a surgical procedure not listed here, we are more than happy to discuss your requirements and the feasibility of performing this through our facility.
Inquire
First Line Phenotyping
The Monash Metabolic Phenotyping Facility can undertake a number of first line phenotyping procedures. Our experienced staff follow standardised procedures that are in line with world leading facilities, are similar to those performed in humans and precede the more complex gold standard in vivo metabolic studies. We can undertake several procedures on your behalf, including:
Body composition analysis (body weight, lean mass, fat mass, bone density).
Whole body glucose metabolism, insulin sensitivity and glucose production.
Exercise endurance and/or exercise capacity ( including gas exchange data)
Pharmacokinetic and half-life characterisation.
Behavioural analysis.
Investigators can utilise our surgical procedures and have the option of choosing one test or a combination of tests to enhance the level of sensitivity for your research project. Should you require us to perform a procedure not listed here, we are more than happy to discuss your requirements and the feasibility of performing your procedure through our facility
Inquire
Gold Standard Metabolic Phenotyping
At the Monash Metabolic Phenotyping Facility, our gold standard metabolic phenotyping tests combine arterial and jugular vein cannulation with radioisotopes to look at whole body insulin action, glucose sensitivity and the metabolic response to treadmill exercise. In addition, the rates of glucose and/or fatty acid uptake by multiple tissue can be assessed.
All tests are performed in conscious and unrestrained mice. These are considered to be the gold standard for examining metabolism in vivo. We can undertake several procedures on your behalf, including:
Hyperinsulinaemic-euglycaemic clamp: assesses whole body insulin action and the ability of insulin to suppress hepatic glucose production. Rates of glucose uptake by multiple tissues can also be assessed.
Hyperglycaemic clamp: assesses blood metabolite and hormone responses to an acute elevation in blood glucose levels.
Hyperinsulinaemic-hypoglycaemic clamp: assesses the counter regulatory response to an acute drop in blood glucose levels via insulin.
Metabolic response to exercise: assesses endogenous glucose production and/or rates of glucose uptake by multiple tissues.
Should you require us to perform a procedure not listed here, we are more than happy to discuss your requirements and the feasibility of performing your procedure through our facility.
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