Monash Macromolecular Crystallisation Facility

Overview of Services

Monash Macromolecular Crystallisation Facility is an automated platform for the high throughput crystallisation of biological macromolecules, operating on a fee-for-service model.

We provide Monash researchers with access to the newest nano-volume crystallisation and imaging technologies using state-of-the-art equipment:

• NT8 (Formulatrix) and

• Mosquito (TTP Labtech) - nano drop dispensing

• Formulator (Formulatrix) - building and dispensing customised optimisation screens using microfluidic technology

• 4 Rock Imagers (Formulatrix) - plate incubators/automated imaging systems

• 2 SONICC crystal detectors (Formulatrix) - using Second Harmonic Generation (SHG) and Ultraviolet Two-Photon Excited Fluorescence (UV-TPEF) for protein crystals detection in sitting drops and LCP

• Prometheus (NanoTemper) - nano DSF – protein thermal unfolding, chemical denaturation and aggregation

Our Services:

• Nano-volume crystallisation screening (vapour diffusion and LCP), followed by automated imaging with remote access to images

• Seeding and additive screening

• Custom screen design and dispensing for optimisation of crystallisation conditions.

• Pre-crystallisation sample evaluation by Differential Scanning Fluorimetry

Leadership

Jamie Rossjohn

Prof/Director

Danuta Maksel

PhD/Manager

Geoffrey Kong

PhD/Senior Technical Officer

Location and hours of operation

Links and Resources

1. Website in construction

Contacts