Welcome!
The Monash Ramaciotti Centre for Cryo-Electron Microscopy (Cryo-EM) is a leading research facility for biological electron microscopy.
Our platform provides expertise in advanced electron microscopy for biomedical discoveries at the molecular and cellular level. We offer a range of biological EM (Bio-EM) techniques from scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to immuno electron microscopy, correlative light and electron microscopy, Cryo-tomography and structure determination using single particle Cryo-EM.
We maintain a suite of advanced electron microscopes (including two Titan KRIOS cryo-TEMs) and the associated specimen preparation equipment, in a specially designed facility. The experienced team in the facility provides expertise, training, and research support in advanced biomedical electron microscopy to researchers from the university, government and industry sectors.
Georg Ramm, Head Cryo EM
georg.ramm@monash.edu
Katie Levick, Manager
katie.levick@monash.edu
| Hours | Location |
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Monday- Friday, 9am-5pm |
15 Innovation Walk, Room G104 |
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Georg Ramm |
Director
|
+61 3 990 51280
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georg.ramm@monash.edu
|
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| Margaret Rzeszutek |
Administrator
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+61 3 990 20878
|
margaret.rzeszutek@monash.edu
|
|
| Katie Levick |
Manager
|
+61 3 990 58905
|
katie.levick@monash.edu
|
| Service list |
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| Name | Description | Price | |
|---|---|---|---|
| Key Instrumentation |
Transmission electron microscopes (TEM) Our platform houses four TEMs, including a TFS Titan KRIOS, a 300keV cryo-TEM designed for automatic data collection. It is equipped with a highly sensitive Falcon III direct electron detector, an energy filter, a phase plate and a Gatan K3 direct electron detector. The KRIOS also has a robotic autoloader capable of holding 12 EM grids under liquid nitrogen conditions. Software packages, EPU for single-particle and Serial EM for tomography, are available for data collection. Our other TEMs include TFS Talos Arctica, a 200keV cryo-TEM dedicated to structural cryo- EM, a TFS Tecnai G2 Spirit (120keV) used for cryo applications and a JEOL JEM-1400Flash for standard TEM.
Focused ion beam scanning electron microscope (FIBSEM) and scanning electron microscope (SEM) Our platform houses Australia's first dedicated cryo-FIBSEM, a TFS Helios G4 UX with a Leica VCT500 cryo stage. The Helios G4 UX is also equipped with an EasyLift NanoManipulator, an Oxford EDS system, and software for Slice & View, CLEM, TEM lamellae preparation, and 3D EDS analysis. Additionally, we also have available a TFS Nova NanoSEM, capable of imaging life science structures in the nanometer range.
Cryo-confocal microscope The Zeiss LSM900 Airyscan 2 confocal microscope is equipped with a Linkam CMS 196 cryo stage. This microscope is dedicated to correlative light and electron microscopy.
Sample preparation suite Our platform also has dedicated equipment to prepare proteins, viruses, bacteria, cells, tissues and small organisms for life sciences EM. This includes dedicated cryopreservation and sample preparation equipment such as a TFS Vitrobot Mark IV freeze plunger, Wohlwend compact 3 high-pressure freezer, Leica UC7/FC7 cryo-ultramicrotomes and a Leica cryo high vacuum coating unit EM ACE600. |
Inquire | |
| Specialist Services |
The Monash Ramaciotti Centre for Cryo-Electron Microscopy(Cryo-EM) is a leading research facility for biological electron microscopy. Our platform provides expertise in advanced electron microscopy for biomedical discoveries at the molecular and cellular level. We offer a range of bio EM techniques from scanning electron microscopy and transmission electron microscopy to immune-electron microscopy, correlative light and electron microscopy, cryo- tomography and protein structure determination and protein characterisation using single-particle cryo-EM. We maintain a suite of advanced electron microscopes, including Australia's first Titan KRIOS currently the most powerful microscope for bio EM.
SPECIALIST SERVICES
Experties Our platform supports Australian researchers by developing and applying advanced imaging techniques to study the life sciences. We conduct research into advanced life sciences EM techniques. Our team also provides training and expertise to Australian and international collaborators on advanced EM projects.
Working with us
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Inquire | |
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| Name | Description | Price | |
| 300keV Dedicated Cryo-Tomography TEM |
The Ramaciotti Centre for Cryo-EM will receive delivery of a 300keV dedicated cryo-tomography TEM for structural and cellular biomedical advances by June 2023. This will enable major drug discovery programs funded through major investments by pharmaceutical companies, alongside breakthroughs in advanced manufacturing, new materials, energy storage and new electronic devices. Procurement of this world-class capability has been possible due to support from the Federal Government (NCRIS), Victorian State Government (VHESIF) and Monash University. Expressions of Interest for access to this new capability are welcomed. Please email cryo-EM@monash.edu |
Inquire | |
