Monash Antibody Technologies Facility

Overview of Services

The Monash Antibody Technologies Facility (MATF) provides services for antibody production, assay development, robotics, high throughput screening and antibody characterisation, including monoclonal antibody sequencing. We use state-of-the-art equipment to support researchers not only from Monash University but elsewhere in Victoria, Australia and world-wide.

The primary focus in the platform is generating high-affinity monoclonal antibodies through advanced discovery techniques and antibody engineering. However, we are keen to help customers with their antibody or laboratory automation challenges. We are happy to develop new antibody screens and encourage our customers to bring their requests to us.

The staff from MATF work with our customers at every step to ensure the needs of each individual project are met and we are proud to follow a Quality Management System that is certified to ISO9001.

MATF and RoboCore Platform provides multiple levels of service for equipment booking:

Assisted Use (staffed hours) a full service where specialist MATF/RoboCore staff will program and run all assays for customers
Self Use (staffed hours) for those customers who wish to run their own assays with little assistance
Self Use (non-staffed hours) for experts who are confident to run the equipment when no MATF staff are around

The MATF Platform operates on a fee for services basis for all services and laboratory access.

MATF is supported by BioPlatforms Australia through the Commonwealth government's National Collaborative Research Infrastructure Strategy (NCRIS)

Manager

Dr. Hayley Ramshaw

61 (3) 9905 8906

Monash ext. 58906

hayley.ramshaw@monash.edu

Staff

Dr Devang Patel	61 (3) 9902 0728	ext. 20728
Bronwyn Briers	61 (3) 9902 0720	ext. 20720
Yeng Chan	61 (3) 9902 4289	ext. 24289

Location and hours of operation

Hours

Location

Monday-Friday, 8:30am-5pm

Building 75, 15 Innovation Walk

Clayton Campus, Monash University

VIC 3800

Links and Resources

Contacts

Name	Role	Phone	Email	Location
Hayley Ramshaw		+61 3 990 58906	hayley.ramshaw@monash.edu	G88, 15 Innovation Walk, Clayton Campus

Service list

Name	Description	Price
ELISA optimisation	ELISA Development: Sandwich ELISA production with your mAbs or using our antibody generation service Do you have a large study that requires ELISA? Let us do the hard work for you. Using our robotic ELISA platforms, MATF can run hundreds of ELISA plates each week using your protocol or we can help you develop a protocol based around your antibody pair. We will consult with you to determine your high throughput ELISA needs and develop a robotic program to suit. We will then run your samples and deliver a comprehensive report of the data in any format you require. Using automated ELISA assays will give you peace of mind that your assays will be run consistently and accurately every time. If you don’t have complementary pairs of antibodies for your ELISA project, why not use our custom antibody service to generate one, or both, of the antibodies needed. Please contact Hayley Ramshaw to discuss your ELISA requirements and/or to obtain a quote	Inquire
Monoclonal antibody sequencing (DNA)	We can isotype and sequence mouse or rat antibodies from hybridoma cell lines generated at the facility as well as cell lines provided by customers. Knowing the sequence of your antibody is essential for mammalian expression, humanisation or for IP protection. The service includes the cloning and sequencing of the light and heavy chain variable regions and typically takes 2-4 weeks. This monoclonal antibody sequencing service requires a frozen cell vial or growing hybridoma culture* for the sequencing of the heavy-chain variable region, (VH), and the light-chain variable region, (VL), of the monoclonal antibody. Data will be analysed using the IMGT/V-Quest program (The International Immunogenetics Information System) and VH and VL sequences are compared to unrearranged germline mouse antibody genes. A project report will be provided at the end of the sequencing in which the VH and VL protein sequences are annotated to highlight framework regions (FRs), and complementary determining regions (CDRs). *sequencing may be performed from a frozen cell pellet or RNA sample but please contact MATF before submitting this as a request	Inquire
Monoclonal antibody sequencing (Protein)	We can determine the amino acid sequence of an unknown antibody using mass spectrometry from bottom-up proteomics techniques with multiple enzyme digests. Antibody protein sequencing does not require the hybridoma cell line or the DNA sequence and we can work with as little as 20µg material. This service requires 20ug material at ~1mg/mL The antibody sample is run on a gel to identify two bands (approx. 20kda and above 50kDa to meet normal expectations for an antibody sample). The sample is then digested with three different enzymes analysed by LCMS and the resulting data processed with the PeaksAB software and manually refined. The final sequences will be provided in a report.	Inquire
SPR determination of mAb pair or binding kinetics	Surface Plasmon Resonance (SPR) is a biosensor technology enabling label-free and real- time measurement of protein-protein interactions. In the capture assay, the ligand of interest binds to the specific antibody that is immobilized on a chip. The kinetics of binding can then be measured. The principle uses of SPR analysis are to identify complimentary binding pairs of antibodies with recombinant antigen or to determine binding kinetics and affinity of antibodies with the parent antigen. Ligand: The ligand should be homogeneous and more than 90% pure when used for covalent binding to the sensor chip. For one immobilization of a molecule using amine coupling, 1-10 µg is normally sufficient. The properties of the ligand, which when known are helpful in experiments, are the molecular mass, iso-electric point and the number of lysine's. Analyte: The analyte concentration of stock solutions should be between 0.1 - 1 mg/ml and free of particles and compounds with large refractive index (e.g. glycerol). The amount needed is about 10 - 100 mg depending on the KD. The properties of the analyte that are helpful in experiments are the molecular mass (should be > 5 kDa for kinetic studies) and pI. The pI should be below nine; otherwise, a lot of non-specific binding is to be expected. Project Timelines: The current turn-around for SPR projects is 4-8 weeks. Some projects may take longer if there is a problem with the proteins or antibodies that requires extra sample manipulation before the analysis can be run. Please contact Hayley Ramshaw for pricing	Inquire

Search available services: View: by category alphabetically

Name	Description	Price
▼ ► Antibody characterisation (3)
Monoclonal antibody sequencing (DNA)	We can isotype and sequence mouse or rat antibodies from hybridoma cell lines generated at the facility as well as cell lines provided by customers. Knowing the sequence of your antibody is essential for mammalian expression, humanisation or for IP protection. The service includes the cloning and sequencing of the light and heavy chain variable regions and typically takes 2-4 weeks. This monoclonal antibody sequencing service requires a frozen cell vial or growing hybridoma culture* for the sequencing of the heavy-chain variable region, (VH), and the light-chain variable region, (VL), of the monoclonal antibody. Data will be analysed using the IMGT/V-Quest program (The International Immunogenetics Information System) and VH and VL sequences are compared to unrearranged germline mouse antibody genes. A project report will be provided at the end of the sequencing in which the VH and VL protein sequences are annotated to highlight framework regions (FRs), and complementary determining regions (CDRs). *sequencing may be performed from a frozen cell pellet or RNA sample but please contact MATF before submitting this as a request	Inquire
Monoclonal antibody sequencing (Protein)	We can determine the amino acid sequence of an unknown antibody using mass spectrometry from bottom-up proteomics techniques with multiple enzyme digests. Antibody protein sequencing does not require the hybridoma cell line or the DNA sequence and we can work with as little as 20µg material. This service requires 20ug material at ~1mg/mL The antibody sample is run on a gel to identify two bands (approx. 20kda and above 50kDa to meet normal expectations for an antibody sample). The sample is then digested with three different enzymes analysed by LCMS and the resulting data processed with the PeaksAB software and manually refined. The final sequences will be provided in a report.	Inquire
SPR determination of mAb pair or binding kinetics	Surface Plasmon Resonance (SPR) is a biosensor technology enabling label-free and real- time measurement of protein-protein interactions. In the capture assay, the ligand of interest binds to the specific antibody that is immobilized on a chip. The kinetics of binding can then be measured. The principle uses of SPR analysis are to identify complimentary binding pairs of antibodies with recombinant antigen or to determine binding kinetics and affinity of antibodies with the parent antigen. Ligand: The ligand should be homogeneous and more than 90% pure when used for covalent binding to the sensor chip. For one immobilization of a molecule using amine coupling, 1-10 µg is normally sufficient. The properties of the ligand, which when known are helpful in experiments, are the molecular mass, iso-electric point and the number of lysine's. Analyte: The analyte concentration of stock solutions should be between 0.1 - 1 mg/ml and free of particles and compounds with large refractive index (e.g. glycerol). The amount needed is about 10 - 100 mg depending on the KD. The properties of the analyte that are helpful in experiments are the molecular mass (should be > 5 kDa for kinetic studies) and pI. The pI should be below nine; otherwise, a lot of non-specific binding is to be expected. Project Timelines: The current turn-around for SPR projects is 4-8 weeks. Some projects may take longer if there is a problem with the proteins or antibodies that requires extra sample manipulation before the analysis can be run. Please contact Hayley Ramshaw for pricing	Inquire
▼ ► ELISA (1)
Name	Description	Price
ELISA optimisation	ELISA Development: Sandwich ELISA production with your mAbs or using our antibody generation service Do you have a large study that requires ELISA? Let us do the hard work for you. Using our robotic ELISA platforms, MATF can run hundreds of ELISA plates each week using your protocol or we can help you develop a protocol based around your antibody pair. We will consult with you to determine your high throughput ELISA needs and develop a robotic program to suit. We will then run your samples and deliver a comprehensive report of the data in any format you require. Using automated ELISA assays will give you peace of mind that your assays will be run consistently and accurately every time. If you don’t have complementary pairs of antibodies for your ELISA project, why not use our custom antibody service to generate one, or both, of the antibodies needed. Please contact Hayley Ramshaw to discuss your ELISA requirements and/or to obtain a quote	Inquire