Biochemistry Imaging Facility

BIOCHEMISTRY IMAGING FACILITY

The Biochemistry Imaging Facility comprises of a number of suites located across the School of Biomedical Sciences specialising in the detection of fluorescence. Some of the main technologies include microscopy, flow cytometry, gel scanning, and microarray detection.

19 Innovation Walk (Bldg 76-G Rms 58-61)

  • Microscopy

    • Nikon TU2000E inverted microscope

      • Fluorescence & Brightfield with phase contrast

      • Image capture with Spot digital camera

      • Blue, Green, UV fluorescent filters

      • Plan Fluor 4X/0.13 PhL DL, Plan Fluor 10X/0.30 Ph1 DL, Plan Fluor ELWD 20X/0.45, Plan Fluor ELWD 40X/0.60, Plan Fluor 100X/1.30 oil Ph3 DLL)

    • Nikon TU2000E inverted microscope

      • Brightfield with phase contrast

      • Image capture with Nikon digital color camera (non fluorescent stains)

      • Plan Fluor 4X/0.13 PhL DL, Plan Fluor 10X/0.30 Ph1 DL, Plan Fluor ELWD 20X/0.45, Plan Fluor ELWD 40X/0.60, Plan Fluor 100X/1.30 oil Ph3 DLL)

    • Olympus IX71 inverted microscope

        • Fluorescence & Brightfield with phase contrast

        • with Eppendorf Microinjection system & Heating stage

        • Image capture with Spot digital camera

        • Fluorescent filter cubes U-MNIBA3, U-MNIBA2, U-MWG2, U-MWIY2, U-MNUA2 Olympus fluorescence filter cubes specs

        • UPlanApo 4X/0.16, CPlanFl 10X/0.30, LUCPlanFl 20X/0.40 RC2 ∞/  ,  LUCPlanFl 40X/0.60 RC3,

 

  • Flow Cytometry

    • Beckman Coulter CyAN triple laser 488nm, 405nm, 635nm (12 parameters including FSC, x2 SSC)

 

  • Gel/blots/slides/Xray films scanning

    • UVP, UViTec, Biorad (simple gel documentation;  gel red, sybr safe, EtBr, etc )
    • Biorad ChemiTouch (Chemiluminescence imaging)
    • GE Healthcare Typhoon5 4 laser LD488, SHG532, LD635, LD685, LD785 (Fluorescence & Radioisotoes/Phosphorimaging, gel band analysis Densitometry)

 

  • Plate readers

    • BMG Pherastar FS (Absorbance, Luminescence, Fluorescence-filter based) (Anisotropy, HTRF, AlphaLisa, AlphaScreen, FRET, BRET etc)  instrument specs & info

 

  • PCR

    • Real Time PCR Qiagen RGQ with HRM 5 channels (green, yellow, orange, red, crimson) & High Resoltuion Melt  instrument specs & info

 

 

 

  • Automated Electrophoresis

    • QIA xcel 96well plate format

 

  • Liquid Handling

    • QIA gility

 

  • DNA/RNA high throughput extractions

    • QIAcube 96well format

 

  • Frozen Tissue Sectioning

    • Leica Cryostat

 

  • Cytospin

    • Shandon cytospin 12 position

 

  • Software Analysis modules on dedicated workstations

    • FlowJo

    • Image Quant IQTL

    • Image J

    • Image Lab

    • CFX Manager

 

15 Innovation Walk (Building 75-L2 & -L3) Imaging Facility-Teaching Lab

  • Plate Readers- BMG Optima (Absorbance, Luminescence, Fluorescence) (filter based)

  • Spectrophotometer- Nanodrop1000 (single channel)

 

39 Rainforest Walk (Bldg 13D-L2 Rms 233 - 238, 201)

  • Microscopy

    • Olympus FV500 & FLIM 5 multi laser line (488nm, 405nm, 635nm)

  • Gel/blot scanning

    • UVP (Gel documentation)

 

Departmental Exclusive equipment

  • Bacterial Shaker Incubators

  • Radiation Laboratory

  • small Gel documentation units

 

Leadership

Irene Erini Hatzinisiriou

Imaging Facility Manager (BCH Safety Officer, BCH Radiation Officer, BCH First Aider & Floor Warden)

ImagingFacility.Biochemistry@monash.edu

 

Location and hours of operation

Hours Location

Facility Hours: 24/7

Staffed Hours: M - F, 9:00am-5:00pm

Biomedicine Discovery Institute

Dept. Biochemistry & Molecular Biology

Bldgs: 19 Innovation Walk (Strip76G58-61) & 35 Rainforest Walk (13D233-234) & 15 Innovation Walk (Strip75 L2 & L3)

Links and Resources

Contacts

Name Role Phone Email Location
Irene Erini Hatzinisiriou
Facility Manager
 

 
ImagingFacility.Biochemistry@monash.edu
 

 

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